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pyrrophenone  (Cayman Chemical)


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    Cayman Chemical pyrrophenone
    Effects of GIVA cPLA 2 inhibitors on cell viability. ( A ) MTT assay for all compounds at 3.5 μΜ. Only <t>pyrrophenone</t> shows significant decrease in cell viability. Statistics obtained by Student’s t -test comparing cells treated with each inhibitor with cells treated with DMSO as vehicle (untreated). # p < 0.0001. DMSO alone had no effect on cell viability at concentrations used (<0.05%, left). Statistics by One-way Anova followed by Dunnett’s multiple comparison test (* p = 0.0224, *** p = 0.0001, **** p < 0.0001). ( B ) IC 50 values obtained following treatment with GK420 or GK484 inhibitors, using nonlinear regression.
    Pyrrophenone, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pyrrophenone/pyrrophenone/pmc12108850-84-0-6
    Average 90 stars, based on 1 article reviews
    pyrrophenone - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "A Lipidomic Approach to Studying the Downregulation of Free Fatty Acids by Cytosolic Phospholipase A 2 Inhibitors"

    Article Title: A Lipidomic Approach to Studying the Downregulation of Free Fatty Acids by Cytosolic Phospholipase A 2 Inhibitors

    Journal: Biomolecules

    doi: 10.3390/biom15050626

    Effects of GIVA cPLA 2 inhibitors on cell viability. ( A ) MTT assay for all compounds at 3.5 μΜ. Only pyrrophenone shows significant decrease in cell viability. Statistics obtained by Student’s t -test comparing cells treated with each inhibitor with cells treated with DMSO as vehicle (untreated). # p < 0.0001. DMSO alone had no effect on cell viability at concentrations used (<0.05%, left). Statistics by One-way Anova followed by Dunnett’s multiple comparison test (* p = 0.0224, *** p = 0.0001, **** p < 0.0001). ( B ) IC 50 values obtained following treatment with GK420 or GK484 inhibitors, using nonlinear regression.
    Figure Legend Snippet: Effects of GIVA cPLA 2 inhibitors on cell viability. ( A ) MTT assay for all compounds at 3.5 μΜ. Only pyrrophenone shows significant decrease in cell viability. Statistics obtained by Student’s t -test comparing cells treated with each inhibitor with cells treated with DMSO as vehicle (untreated). # p < 0.0001. DMSO alone had no effect on cell viability at concentrations used (<0.05%, left). Statistics by One-way Anova followed by Dunnett’s multiple comparison test (* p = 0.0224, *** p = 0.0001, **** p < 0.0001). ( B ) IC 50 values obtained following treatment with GK420 or GK484 inhibitors, using nonlinear regression.

    Techniques Used: MTT Assay, Comparison

    Change in intracellular FFAs upon treatment of SH-SY5Y cells with pyrrophenone and CAY10502.
    Figure Legend Snippet: Change in intracellular FFAs upon treatment of SH-SY5Y cells with pyrrophenone and CAY10502.

    Techniques Used:

    Related Articles

    Generated:

    Article Title: Systematic Single Cell Pathway Analysis (SCPA) to characterize early T cell activation
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human CD4-PE Biolegend Cat#980804; RRID:AB_2616622 Anti-human CD8-PercpCy5.5 Biolegend Cat#344709; RRID:AB_2044009 Anti-human CD56-APC Biolegend Cat#981204; RRID:AB_2715759 Anti-human CD45RA-FITC Biolegend Cat#983002; RRID:AB_2650650 Anti-human CD45RO-BV421 Biolegend Cat#304223; RRID:AB_2563817 Live-dead near IR Thermofisher Cat#L10119 Anti-human CD4-Pacific Blue Biolegend Cat#317429; RRID:AB_1595438 Anti-human CD69-APC Biolegend Cat#310909; RRID:AB_314844 Anti-human CD45RA-APC Biolegend Cat#304111; RRID:AB_314415 Anti-human CD45RO-FITC Biolegend Cat#304204; RRID:AB_314420 Anti-human DEFA1 R&D Systems Cat#AF8198-SP anti-sheep IgG-NL557 R&D Systems Cat#NL010; RRID:AB_884220 Anti-human CD3 In house generated from OKT3 hybridoma Anti-human CD28 eBioscience Cat#16-0289-81; RRID:AB_468926 Human IFNγ-APC secretion assay Miltenyi Cat#130-054-202 Bacterial and virus strains Biological samples Chemicals, peptides, and recombinant proteins Pyrrophenone Cayman Chemical Cat#13294 Lymphoprep separation medium Corning Cat#25-072-CV Ficoll-Hypaque density gradient MP Biomedicals Cat#02160003-CF CryoStor CS5 freezing medium Biolife Solutions Cat#205373 Alt-R S.p. .. Cas9 Nuclease V3 Integrated DNA Technologies Cat#1081059 Oligomycin Sigma Aldrich Cat#O4876 Carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone Sigma Aldrich Cat#C2920 Rotenone Sigma Aldrich Cat#R8875 Human recombinant IL-2 Peprotech Cat#200-02 ACK lysis buffer Life Technologies Cat#A1049201 Critical commercial assays VeriKine-HS Human IFNα all subtype TCM ELISA PLB assay science Cat#41115-1 Legendplex inflammation mix and match Biologend N/A NEBNext Ultra II RNA Library Prep Kit New England Biolabs Cat#E7775 Human CD8 + T cell Isolation Kit Miltenyi Biotech Cat#130-096-495 FOXP3/Transcription factor staining kit eBioscience Cat#00-5523-00 Stem Cell Technologies EasySep Mouse CD4+ T Cell Isolation Kit Stemcell Technologies Cat#19852 P3 primary Cell 4D-Nucleofector X kit Lonza Cat#V4XP-3032 Quick-DNATM Microprep kit Zymo Research Cat#D3021 Q5 ® Hot Start High-Fidelity 2X Master Mix New England Biolabs Cat#M0494 DNA Clean & ConcentratorTM Zymo Research Cat# D4004 EasySep Human CD4 + T Cell Isolation Kit Stemcell Technologies Cat#17952 Deposited data scRNA-seq on stimulated T cell populations This paper tbd Bulk RNA-seq on Ifnar1 −/− mice This paper tbd Experimental models: Cell lines Experimental models: Organisms/strains C57BL/6 WT mice Jackson Laboratory RRID:IMSR_JAX:000664 B6(Cg)-Ifnar1tm1.2Ees/J mice Jackson Laboratory RRID: IMSR_JAX:028288 Oligonucleotides Alt-R CRISPR-Cas9 tracrRNA Integrated DNA Technologies Cat#1072534 Non-targeting control gRNA addgene Cat# BRDN0001149198 Recombinant DNA Software and algorithms FastQC Babraham Bioinformatics https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ ; RRID:SCR_014583 MultiQC v1.12 1 https://multiqc.info ; RRID:SCR_014982 Trim Galore!

    Virus:

    Article Title: Systematic Single Cell Pathway Analysis (SCPA) to characterize early T cell activation
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human CD4-PE Biolegend Cat#980804; RRID:AB_2616622 Anti-human CD8-PercpCy5.5 Biolegend Cat#344709; RRID:AB_2044009 Anti-human CD56-APC Biolegend Cat#981204; RRID:AB_2715759 Anti-human CD45RA-FITC Biolegend Cat#983002; RRID:AB_2650650 Anti-human CD45RO-BV421 Biolegend Cat#304223; RRID:AB_2563817 Live-dead near IR Thermofisher Cat#L10119 Anti-human CD4-Pacific Blue Biolegend Cat#317429; RRID:AB_1595438 Anti-human CD69-APC Biolegend Cat#310909; RRID:AB_314844 Anti-human CD45RA-APC Biolegend Cat#304111; RRID:AB_314415 Anti-human CD45RO-FITC Biolegend Cat#304204; RRID:AB_314420 Anti-human DEFA1 R&D Systems Cat#AF8198-SP anti-sheep IgG-NL557 R&D Systems Cat#NL010; RRID:AB_884220 Anti-human CD3 In house generated from OKT3 hybridoma Anti-human CD28 eBioscience Cat#16-0289-81; RRID:AB_468926 Human IFNγ-APC secretion assay Miltenyi Cat#130-054-202 Bacterial and virus strains Biological samples Chemicals, peptides, and recombinant proteins Pyrrophenone Cayman Chemical Cat#13294 Lymphoprep separation medium Corning Cat#25-072-CV Ficoll-Hypaque density gradient MP Biomedicals Cat#02160003-CF CryoStor CS5 freezing medium Biolife Solutions Cat#205373 Alt-R S.p. .. Cas9 Nuclease V3 Integrated DNA Technologies Cat#1081059 Oligomycin Sigma Aldrich Cat#O4876 Carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone Sigma Aldrich Cat#C2920 Rotenone Sigma Aldrich Cat#R8875 Human recombinant IL-2 Peprotech Cat#200-02 ACK lysis buffer Life Technologies Cat#A1049201 Critical commercial assays VeriKine-HS Human IFNα all subtype TCM ELISA PLB assay science Cat#41115-1 Legendplex inflammation mix and match Biologend N/A NEBNext Ultra II RNA Library Prep Kit New England Biolabs Cat#E7775 Human CD8 + T cell Isolation Kit Miltenyi Biotech Cat#130-096-495 FOXP3/Transcription factor staining kit eBioscience Cat#00-5523-00 Stem Cell Technologies EasySep Mouse CD4+ T Cell Isolation Kit Stemcell Technologies Cat#19852 P3 primary Cell 4D-Nucleofector X kit Lonza Cat#V4XP-3032 Quick-DNATM Microprep kit Zymo Research Cat#D3021 Q5 ® Hot Start High-Fidelity 2X Master Mix New England Biolabs Cat#M0494 DNA Clean & ConcentratorTM Zymo Research Cat# D4004 EasySep Human CD4 + T Cell Isolation Kit Stemcell Technologies Cat#17952 Deposited data scRNA-seq on stimulated T cell populations This paper tbd Bulk RNA-seq on Ifnar1 −/− mice This paper tbd Experimental models: Cell lines Experimental models: Organisms/strains C57BL/6 WT mice Jackson Laboratory RRID:IMSR_JAX:000664 B6(Cg)-Ifnar1tm1.2Ees/J mice Jackson Laboratory RRID: IMSR_JAX:028288 Oligonucleotides Alt-R CRISPR-Cas9 tracrRNA Integrated DNA Technologies Cat#1072534 Non-targeting control gRNA addgene Cat# BRDN0001149198 Recombinant DNA Software and algorithms FastQC Babraham Bioinformatics https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ ; RRID:SCR_014583 MultiQC v1.12 1 https://multiqc.info ; RRID:SCR_014982 Trim Galore!

    Recombinant:

    Article Title: Systematic Single Cell Pathway Analysis (SCPA) to characterize early T cell activation
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human CD4-PE Biolegend Cat#980804; RRID:AB_2616622 Anti-human CD8-PercpCy5.5 Biolegend Cat#344709; RRID:AB_2044009 Anti-human CD56-APC Biolegend Cat#981204; RRID:AB_2715759 Anti-human CD45RA-FITC Biolegend Cat#983002; RRID:AB_2650650 Anti-human CD45RO-BV421 Biolegend Cat#304223; RRID:AB_2563817 Live-dead near IR Thermofisher Cat#L10119 Anti-human CD4-Pacific Blue Biolegend Cat#317429; RRID:AB_1595438 Anti-human CD69-APC Biolegend Cat#310909; RRID:AB_314844 Anti-human CD45RA-APC Biolegend Cat#304111; RRID:AB_314415 Anti-human CD45RO-FITC Biolegend Cat#304204; RRID:AB_314420 Anti-human DEFA1 R&D Systems Cat#AF8198-SP anti-sheep IgG-NL557 R&D Systems Cat#NL010; RRID:AB_884220 Anti-human CD3 In house generated from OKT3 hybridoma Anti-human CD28 eBioscience Cat#16-0289-81; RRID:AB_468926 Human IFNγ-APC secretion assay Miltenyi Cat#130-054-202 Bacterial and virus strains Biological samples Chemicals, peptides, and recombinant proteins Pyrrophenone Cayman Chemical Cat#13294 Lymphoprep separation medium Corning Cat#25-072-CV Ficoll-Hypaque density gradient MP Biomedicals Cat#02160003-CF CryoStor CS5 freezing medium Biolife Solutions Cat#205373 Alt-R S.p. .. Cas9 Nuclease V3 Integrated DNA Technologies Cat#1081059 Oligomycin Sigma Aldrich Cat#O4876 Carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone Sigma Aldrich Cat#C2920 Rotenone Sigma Aldrich Cat#R8875 Human recombinant IL-2 Peprotech Cat#200-02 ACK lysis buffer Life Technologies Cat#A1049201 Critical commercial assays VeriKine-HS Human IFNα all subtype TCM ELISA PLB assay science Cat#41115-1 Legendplex inflammation mix and match Biologend N/A NEBNext Ultra II RNA Library Prep Kit New England Biolabs Cat#E7775 Human CD8 + T cell Isolation Kit Miltenyi Biotech Cat#130-096-495 FOXP3/Transcription factor staining kit eBioscience Cat#00-5523-00 Stem Cell Technologies EasySep Mouse CD4+ T Cell Isolation Kit Stemcell Technologies Cat#19852 P3 primary Cell 4D-Nucleofector X kit Lonza Cat#V4XP-3032 Quick-DNATM Microprep kit Zymo Research Cat#D3021 Q5 ® Hot Start High-Fidelity 2X Master Mix New England Biolabs Cat#M0494 DNA Clean & ConcentratorTM Zymo Research Cat# D4004 EasySep Human CD4 + T Cell Isolation Kit Stemcell Technologies Cat#17952 Deposited data scRNA-seq on stimulated T cell populations This paper tbd Bulk RNA-seq on Ifnar1 −/− mice This paper tbd Experimental models: Cell lines Experimental models: Organisms/strains C57BL/6 WT mice Jackson Laboratory RRID:IMSR_JAX:000664 B6(Cg)-Ifnar1tm1.2Ees/J mice Jackson Laboratory RRID: IMSR_JAX:028288 Oligonucleotides Alt-R CRISPR-Cas9 tracrRNA Integrated DNA Technologies Cat#1072534 Non-targeting control gRNA addgene Cat# BRDN0001149198 Recombinant DNA Software and algorithms FastQC Babraham Bioinformatics https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ ; RRID:SCR_014583 MultiQC v1.12 1 https://multiqc.info ; RRID:SCR_014982 Trim Galore!

    Incubation:

    Article Title: Cannabidiol acts as molecular switch in innate immune cells to promote the biosynthesis of inflammation-resolving lipid mediators
    Article Snippet: .. To investigate the influence of different compounds on the effect of CBD, M2-MDM (10 6 /mL) were pretreated with pyrrophenone (2 μM; Cayman Chemical/Biomol GmbH), BLX-3887 (1 μM; Cayman Chemical/Biomol), skepinone-L (3 μM; Cayman Chemical/Biomol), U-0126 (10 μM; Cayman Chemical/Biomol), BAPTA (20 μM; Cayman Chemical/Biomol), EDTA (1 mM), or a mixture of AA, EPA, and DHA (1 μM; Cayman Chemical/Biomol GmbH, Hamburg, Germany) for 15 min prior to incubation with CBD for 90 min. ..

    Article Title: Cannabidiol acts as molecular switch in innate immune cells to promote the biosynthesis of inflammation-resolving lipid mediators.
    Article Snippet: In brief Peltner et al. reveal cannabidiol from cannabis as lipid mediator classswitching agent that stimulates innate immune cells for producing specialized pro-resolving mediators and suppresses pro-inflammatory leukotriene formation.. Cannabidiol activates 15-lipoxygenase-1 via an allosteric site which causes SPM production in humanmacrophages and in zymosan-induced murine peritonitis in vivo.

    Modification:

    Article Title: Deficiency of Group IVA Phospholipase A 2 in Collagen-Producing Cells Alleviates the Aggravated Hepatic Fibrosis in High-Fat Diet-Fed Mice after Returning to a Normal Diet.
    Article Snippet: .. TWNT1 (immortalized human hepatic stellate cell line; the Japanese Collection of Research Bioresources Cell Bank, Tokyo, Japan), a model of activated HSCs, was maintained in low glucose-Dulbecco’s modified Eagle medium (DMEM) with 10% fetal bovine serum and antibiotics, and treated with pyrrophenone (Cayman Chemical Co., Ann Arbor, MI, U.S.A.), a specific IVA-PLA2 inhibitor,18) for 24 h. Serum Biomarker Measurements Blood samples collected from the inferior vena cava were left to stand for 1 h. The serum was prepared by centrifugation at 10000 × g for 10 min at room temperature. .. The activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured using Transaminase C II-Test kits (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan).

    Biomarker Discovery:

    Article Title: Deficiency of Group IVA Phospholipase A 2 in Collagen-Producing Cells Alleviates the Aggravated Hepatic Fibrosis in High-Fat Diet-Fed Mice after Returning to a Normal Diet.
    Article Snippet: .. TWNT1 (immortalized human hepatic stellate cell line; the Japanese Collection of Research Bioresources Cell Bank, Tokyo, Japan), a model of activated HSCs, was maintained in low glucose-Dulbecco’s modified Eagle medium (DMEM) with 10% fetal bovine serum and antibiotics, and treated with pyrrophenone (Cayman Chemical Co., Ann Arbor, MI, U.S.A.), a specific IVA-PLA2 inhibitor,18) for 24 h. Serum Biomarker Measurements Blood samples collected from the inferior vena cava were left to stand for 1 h. The serum was prepared by centrifugation at 10000 × g for 10 min at room temperature. .. The activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured using Transaminase C II-Test kits (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan).

    Centrifugation:

    Article Title: Deficiency of Group IVA Phospholipase A 2 in Collagen-Producing Cells Alleviates the Aggravated Hepatic Fibrosis in High-Fat Diet-Fed Mice after Returning to a Normal Diet.
    Article Snippet: .. TWNT1 (immortalized human hepatic stellate cell line; the Japanese Collection of Research Bioresources Cell Bank, Tokyo, Japan), a model of activated HSCs, was maintained in low glucose-Dulbecco’s modified Eagle medium (DMEM) with 10% fetal bovine serum and antibiotics, and treated with pyrrophenone (Cayman Chemical Co., Ann Arbor, MI, U.S.A.), a specific IVA-PLA2 inhibitor,18) for 24 h. Serum Biomarker Measurements Blood samples collected from the inferior vena cava were left to stand for 1 h. The serum was prepared by centrifugation at 10000 × g for 10 min at room temperature. .. The activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured using Transaminase C II-Test kits (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan).



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    Ferroptosis inhibition by CEPT1 is partially dependent on phospholipases. (A–D) Pulldown assays were performed with S protein beads (SFB-tagged proteins were used as the baits), and the indicated proteins were detected by WB. (E) Cell death was measured by propidium iodide (PI) staining in control (sgControl), DDHD1 -knockout, or DDHD2 -knockout (sg1/2) 786-O cells overexpressing CEPT1 or transfected with empty vector (EV) treated with 50 nmol/L RSL3 for 24 h. (F) Cell death was measured by PI staining in control (shControl) and ABHD3 -knockdown (sh1/3) 786-O cells overexpressing CEPT1 or transfected with EV treated with 50 nmol/L RSL3 for 24 h. (G and H) Cell death was measured by PI staining in control (sgControl) and PLAA -knockout (sg1/2) 786-O cells overexpressing CEPT1 or transfected with EV treated with 50 nmol/L RSL3 (G) or 10 µmol/L erastin (H) for 24 h. (I and J) Cell death was measured by PI staining in 786-O cells overexpressing CEPT1 or transfected with EV treated with DMSO, 50 nmol/L RSL3 (I), or 10 µmol/L erastin (J) with 1 µmol/L <t>pyrrophenone</t> (PY) or indicated combinations for 24 h. (K) PLA2 enzyme activities were measured in 786-O cells overexpressing CEPT1 WT or indicated mutants or transfected with an empty vector (EV).
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    Image Search Results


    Effects of GIVA cPLA 2 inhibitors on cell viability. ( A ) MTT assay for all compounds at 3.5 μΜ. Only pyrrophenone shows significant decrease in cell viability. Statistics obtained by Student’s t -test comparing cells treated with each inhibitor with cells treated with DMSO as vehicle (untreated). # p < 0.0001. DMSO alone had no effect on cell viability at concentrations used (<0.05%, left). Statistics by One-way Anova followed by Dunnett’s multiple comparison test (* p = 0.0224, *** p = 0.0001, **** p < 0.0001). ( B ) IC 50 values obtained following treatment with GK420 or GK484 inhibitors, using nonlinear regression.

    Journal: Biomolecules

    Article Title: A Lipidomic Approach to Studying the Downregulation of Free Fatty Acids by Cytosolic Phospholipase A 2 Inhibitors

    doi: 10.3390/biom15050626

    Figure Lengend Snippet: Effects of GIVA cPLA 2 inhibitors on cell viability. ( A ) MTT assay for all compounds at 3.5 μΜ. Only pyrrophenone shows significant decrease in cell viability. Statistics obtained by Student’s t -test comparing cells treated with each inhibitor with cells treated with DMSO as vehicle (untreated). # p < 0.0001. DMSO alone had no effect on cell viability at concentrations used (<0.05%, left). Statistics by One-way Anova followed by Dunnett’s multiple comparison test (* p = 0.0224, *** p = 0.0001, **** p < 0.0001). ( B ) IC 50 values obtained following treatment with GK420 or GK484 inhibitors, using nonlinear regression.

    Article Snippet: Pyrrophenone and CAY10502 were purchased from Cayman Chemical Company (>98%, Ann Arbor, MI, USA).

    Techniques: MTT Assay, Comparison

    Change in intracellular FFAs upon treatment of SH-SY5Y cells with pyrrophenone and CAY10502.

    Journal: Biomolecules

    Article Title: A Lipidomic Approach to Studying the Downregulation of Free Fatty Acids by Cytosolic Phospholipase A 2 Inhibitors

    doi: 10.3390/biom15050626

    Figure Lengend Snippet: Change in intracellular FFAs upon treatment of SH-SY5Y cells with pyrrophenone and CAY10502.

    Article Snippet: Pyrrophenone and CAY10502 were purchased from Cayman Chemical Company (>98%, Ann Arbor, MI, USA).

    Techniques:

    Ferroptosis inhibition by CEPT1 is partially dependent on phospholipases. (A–D) Pulldown assays were performed with S protein beads (SFB-tagged proteins were used as the baits), and the indicated proteins were detected by WB. (E) Cell death was measured by propidium iodide (PI) staining in control (sgControl), DDHD1 -knockout, or DDHD2 -knockout (sg1/2) 786-O cells overexpressing CEPT1 or transfected with empty vector (EV) treated with 50 nmol/L RSL3 for 24 h. (F) Cell death was measured by PI staining in control (shControl) and ABHD3 -knockdown (sh1/3) 786-O cells overexpressing CEPT1 or transfected with EV treated with 50 nmol/L RSL3 for 24 h. (G and H) Cell death was measured by PI staining in control (sgControl) and PLAA -knockout (sg1/2) 786-O cells overexpressing CEPT1 or transfected with EV treated with 50 nmol/L RSL3 (G) or 10 µmol/L erastin (H) for 24 h. (I and J) Cell death was measured by PI staining in 786-O cells overexpressing CEPT1 or transfected with EV treated with DMSO, 50 nmol/L RSL3 (I), or 10 µmol/L erastin (J) with 1 µmol/L pyrrophenone (PY) or indicated combinations for 24 h. (K) PLA2 enzyme activities were measured in 786-O cells overexpressing CEPT1 WT or indicated mutants or transfected with an empty vector (EV).

    Journal: Protein & Cell

    Article Title: Proteomic analysis of ferroptosis pathways reveals a role of CEPT1 in suppressing ferroptosis

    doi: 10.1093/procel/pwae004

    Figure Lengend Snippet: Ferroptosis inhibition by CEPT1 is partially dependent on phospholipases. (A–D) Pulldown assays were performed with S protein beads (SFB-tagged proteins were used as the baits), and the indicated proteins were detected by WB. (E) Cell death was measured by propidium iodide (PI) staining in control (sgControl), DDHD1 -knockout, or DDHD2 -knockout (sg1/2) 786-O cells overexpressing CEPT1 or transfected with empty vector (EV) treated with 50 nmol/L RSL3 for 24 h. (F) Cell death was measured by PI staining in control (shControl) and ABHD3 -knockdown (sh1/3) 786-O cells overexpressing CEPT1 or transfected with EV treated with 50 nmol/L RSL3 for 24 h. (G and H) Cell death was measured by PI staining in control (sgControl) and PLAA -knockout (sg1/2) 786-O cells overexpressing CEPT1 or transfected with EV treated with 50 nmol/L RSL3 (G) or 10 µmol/L erastin (H) for 24 h. (I and J) Cell death was measured by PI staining in 786-O cells overexpressing CEPT1 or transfected with EV treated with DMSO, 50 nmol/L RSL3 (I), or 10 µmol/L erastin (J) with 1 µmol/L pyrrophenone (PY) or indicated combinations for 24 h. (K) PLA2 enzyme activities were measured in 786-O cells overexpressing CEPT1 WT or indicated mutants or transfected with an empty vector (EV).

    Article Snippet: Ferroptosis inducer (1S,3R)-RSL3 (#19288), erastin (#17754), arachidonic acid (#90010), and pyrrophenone (#13294) were from Cayman Chemical.

    Techniques: Inhibition, Staining, Control, Knock-Out, Transfection, Plasmid Preparation, Knockdown